Diversity in CMA- and DAPI-Banded Regions in Ten Germplasms of <i>Crotalaria</i> from Bangladesh

Ten germplasms of three Crotalaria species viz. C. pallida (Acc. No. 4250, 4803, 4805, 4806, and 4807), incana 4790, 4801, 4804, 4809), juncea (Local) were investigated cytogenetically through Chromomycin A3 (CMA) 4′-6 diamidino-2-phenylindole (DAPI) fluorescent bandings. After CMA-banding, 10 showed 31 centromeric 34 terminal bands which indicated a tendency accumulating GC-rich sequences at regions or chromosomal ends. The number DAPI was less than that the CMA found in different Crotalaria. Maximum DAPI-bands AT-rich Few chromosomes Acc. 4803 4804 could be used as marker respective due to their unique DAPI-banding pattern. Fluorescent banding revealed occurrence genomic alteration within these germplasms. In 4790 incana, 2n=17 observed indicating its aneuploid origin correlates with phenotypic features like seedless pod formation. diversity CMA- DAPI-banded ten from Bangladesh can help further breeding programs.